While Spider-Man relies on his humor and quips to irritate his foes and throw them off-balance, he once tried his hand at being more intimidating by using a voice inspired by Christian Bale's Batman in the Dark Knight trilogy. While he hilariously fails in his first attempt, the joke serves as a confirmation that DC Comics and its heroes exist in the Marvel Universe in a fictional sense. Furthermore, the fact that Peter Parker must have sat down and watched The Dark Knight at one point is pretty funny.
Featured in 2009's Amazing Spider-Man #589 from writer Fred Van Lente and artist Paulo Siqueira, Spider-Man is seen investigating the Russian mob after his Aunt May was harassed working at a FEAST center. However, the issue also saw the return of the villain Spot who had no love for the Russians either, using his powers from the Dark Dimension to get the drop on the gangsters with deadly results. However, the gang's leader manipulates Spider-Man into rescuing him, knowing that he would be too good of a hero to leave him to die.
After knocking out the gang boss and carrying him to safety, Spider-Man wishes that he could be more intimidating. Thinking that it might help him be more effective as a masked vigilante, Peter Parker attempts to mimic the deep, gravelly voice of Christian Bale's Batman from The Dark Knight films. While he doesn't have much luck, it's still an incredibly hilarious scene where one of Marvel's most popular heroes tries to emulate one of DC's.
Spider-Man actually attempts the Dark Knight voice twice in this classic issue, the second time having more success instilling fear into one of the other goons. He even remarks that criminals truly are "a superstitious and cowardly lot" as Batman is fond of saying in DC Comics. Furthermore, while Marvel and DC have had their crossovers in the past where the DC Universe is an alternate dimension, it seems as though they also exist as a fictional part of the Marvel Universe's continuity.
In the same vein, Marvel's Black Cat made a reference to Adam West's Batman series from the 1960s in more recent Spider-Man comics, proving the same concept that DC Comics exists as a fiction in the Marvel Universe. Even the MCU references both Superman and Batman in the film Eternals. Regardless, the fact that Spider-Man canonically attempted to use a Christian Bale-inspired Batman voice to scare his enemies is a truly great and noteworthy fact about the Webslinger in Marvel Comics.